lv vector backbone prrlsin cppt pgk egfp wpre Search Results


prrlsin.cppt.pgkgfp.wpre lentiviral vector  (Addgene inc)
90
Addgene inc prrlsin.cppt.pgkgfp.wpre lentiviral vector
Prrlsin.Cppt.Pgkgfp.Wpre Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prrlsin cppt pgk gfp wpre vector  (Addgene inc)
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Addgene inc prrlsin cppt pgk gfp wpre vector
Prrlsin Cppt Pgk Gfp Wpre Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prrlsin cppt pgk wpre vectors  (Thermo Fisher)
86
Thermo Fisher prrlsin cppt pgk wpre vectors
Prrlsin Cppt Pgk Wpre Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prrlsin.cppt.pgk-gfp.wpre  (Addgene inc)
90
Addgene inc prrlsin.cppt.pgk-gfp.wpre
Prrlsin.Cppt.Pgk Gfp.Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prrlsin.cppt.pgk-gfp.wpre/product/Addgene inc
Average 90 stars, based on 1 article reviews
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lentiviral expression vectors prrlsin.cppt.pgk-gfp.wpre  (Addgene inc)
90
Addgene inc lentiviral expression vectors prrlsin.cppt.pgk-gfp.wpre
Lentiviral Expression Vectors Prrlsin.Cppt.Pgk Gfp.Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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fbxw7 overexpression lentiviral vector (prrlsin-cppt-sffv-mcs-3flag-e2a-egfp-sv40-puromycin  (Genechem)
90
Genechem fbxw7 overexpression lentiviral vector (prrlsin-cppt-sffv-mcs-3flag-e2a-egfp-sv40-puromycin
<t>FBXW7</t> participated in the ubiquitination and degradation of VDAC3. (A,B) Top 10 E3 ligases of VDAC3. (C) Whole cell lysates were prepared and subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. A FBXW7 antibody was used for IB. (D) Whole cell lysates were prepared and subjected to immunoprecipitation with either the FBXW7 antibody or normal IgG antibody. A VDAC3 antibody was used for IB. (E) Comparison of expression levels of FBXW7 in Jurkat, CCRF-CEM, and Reh cells by IB. The expression of β-actin was examined as a control. (F) Histogram of the relative expression levels of FBXW7 under different treatments. Results are presented as means ± SD of three independent experiments. * p < 0.05, ** p < 0.01.
Fbxw7 Overexpression Lentiviral Vector (Prrlsin Cppt Sffv Mcs 3flag E2a Egfp Sv40 Puromycin, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
fbxw7 overexpression lentiviral vector (prrlsin-cppt-sffv-mcs-3flag-e2a-egfp-sv40-puromycin - by Bioz Stars, 2026-03
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lentiviral luciferase vector prrlsin.cppt.rfpl4b.luciferase  (Addgene inc)
90
Addgene inc lentiviral luciferase vector prrlsin.cppt.rfpl4b.luciferase
<t>FBXW7</t> participated in the ubiquitination and degradation of VDAC3. (A,B) Top 10 E3 ligases of VDAC3. (C) Whole cell lysates were prepared and subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. A FBXW7 antibody was used for IB. (D) Whole cell lysates were prepared and subjected to immunoprecipitation with either the FBXW7 antibody or normal IgG antibody. A VDAC3 antibody was used for IB. (E) Comparison of expression levels of FBXW7 in Jurkat, CCRF-CEM, and Reh cells by IB. The expression of β-actin was examined as a control. (F) Histogram of the relative expression levels of FBXW7 under different treatments. Results are presented as means ± SD of three independent experiments. * p < 0.05, ** p < 0.01.
Lentiviral Luciferase Vector Prrlsin.Cppt.Rfpl4b.Luciferase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prrlsin cppt pgk gfp wpre plasmid  (Addgene inc)
93
Addgene inc prrlsin cppt pgk gfp wpre plasmid
<t>FBXW7</t> participated in the ubiquitination and degradation of VDAC3. (A,B) Top 10 E3 ligases of VDAC3. (C) Whole cell lysates were prepared and subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. A FBXW7 antibody was used for IB. (D) Whole cell lysates were prepared and subjected to immunoprecipitation with either the FBXW7 antibody or normal IgG antibody. A VDAC3 antibody was used for IB. (E) Comparison of expression levels of FBXW7 in Jurkat, CCRF-CEM, and Reh cells by IB. The expression of β-actin was examined as a control. (F) Histogram of the relative expression levels of FBXW7 under different treatments. Results are presented as means ± SD of three independent experiments. * p < 0.05, ** p < 0.01.
Prrlsin Cppt Pgk Gfp Wpre Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prrlsin cppt pgk gfp wpre plasmid/product/Addgene inc
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generation lentiviral expression vector prrlsin cppt pgk gfp wpre  (Addgene inc)
92
Addgene inc generation lentiviral expression vector prrlsin cppt pgk gfp wpre
<t>FBXW7</t> participated in the ubiquitination and degradation of VDAC3. (A,B) Top 10 E3 ligases of VDAC3. (C) Whole cell lysates were prepared and subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. A FBXW7 antibody was used for IB. (D) Whole cell lysates were prepared and subjected to immunoprecipitation with either the FBXW7 antibody or normal IgG antibody. A VDAC3 antibody was used for IB. (E) Comparison of expression levels of FBXW7 in Jurkat, CCRF-CEM, and Reh cells by IB. The expression of β-actin was examined as a control. (F) Histogram of the relative expression levels of FBXW7 under different treatments. Results are presented as means ± SD of three independent experiments. * p < 0.05, ** p < 0.01.
Generation Lentiviral Expression Vector Prrlsin Cppt Pgk Gfp Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/generation lentiviral expression vector prrlsin cppt pgk gfp wpre/product/Addgene inc
Average 92 stars, based on 1 article reviews
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lentiviral vector prrlsin cppt rfpl4b luciferase wpre  (Addgene inc)
93
Addgene inc lentiviral vector prrlsin cppt rfpl4b luciferase wpre
<t>FBXW7</t> participated in the ubiquitination and degradation of VDAC3. (A,B) Top 10 E3 ligases of VDAC3. (C) Whole cell lysates were prepared and subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. A FBXW7 antibody was used for IB. (D) Whole cell lysates were prepared and subjected to immunoprecipitation with either the FBXW7 antibody or normal IgG antibody. A VDAC3 antibody was used for IB. (E) Comparison of expression levels of FBXW7 in Jurkat, CCRF-CEM, and Reh cells by IB. The expression of β-actin was examined as a control. (F) Histogram of the relative expression levels of FBXW7 under different treatments. Results are presented as means ± SD of three independent experiments. * p < 0.05, ** p < 0.01.
Lentiviral Vector Prrlsin Cppt Rfpl4b Luciferase Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lentiviral vector prrlsin cppt rfpl4b luciferase wpre/product/Addgene inc
Average 93 stars, based on 1 article reviews
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gv643 (prrlsin-cppt-sffv-mcs-3flag-e2a-egfp-sv40-puromycin) vector  (Genechem)
90
Genechem gv643 (prrlsin-cppt-sffv-mcs-3flag-e2a-egfp-sv40-puromycin) vector
<t>FBXW7</t> participated in the ubiquitination and degradation of VDAC3. (A,B) Top 10 E3 ligases of VDAC3. (C) Whole cell lysates were prepared and subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. A FBXW7 antibody was used for IB. (D) Whole cell lysates were prepared and subjected to immunoprecipitation with either the FBXW7 antibody or normal IgG antibody. A VDAC3 antibody was used for IB. (E) Comparison of expression levels of FBXW7 in Jurkat, CCRF-CEM, and Reh cells by IB. The expression of β-actin was examined as a control. (F) Histogram of the relative expression levels of FBXW7 under different treatments. Results are presented as means ± SD of three independent experiments. * p < 0.05, ** p < 0.01.
Gv643 (Prrlsin Cppt Sffv Mcs 3flag E2a Egfp Sv40 Puromycin) Vector, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gv643 (prrlsin-cppt-sffv-mcs-3flag-e2a-egfp-sv40-puromycin) vector/product/Genechem
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gv643 (prrlsin-cppt-sffv-mcs-3flag-e2a-egfp-sv40-puromycin) vector - by Bioz Stars, 2026-03
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prrlsin-cppt.ef1α  (Addgene inc)
90
Addgene inc prrlsin-cppt.ef1α
<t>FBXW7</t> participated in the ubiquitination and degradation of VDAC3. (A,B) Top 10 E3 ligases of VDAC3. (C) Whole cell lysates were prepared and subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. A FBXW7 antibody was used for IB. (D) Whole cell lysates were prepared and subjected to immunoprecipitation with either the FBXW7 antibody or normal IgG antibody. A VDAC3 antibody was used for IB. (E) Comparison of expression levels of FBXW7 in Jurkat, CCRF-CEM, and Reh cells by IB. The expression of β-actin was examined as a control. (F) Histogram of the relative expression levels of FBXW7 under different treatments. Results are presented as means ± SD of three independent experiments. * p < 0.05, ** p < 0.01.
Prrlsin Cppt.Ef1α, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FBXW7 participated in the ubiquitination and degradation of VDAC3. (A,B) Top 10 E3 ligases of VDAC3. (C) Whole cell lysates were prepared and subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. A FBXW7 antibody was used for IB. (D) Whole cell lysates were prepared and subjected to immunoprecipitation with either the FBXW7 antibody or normal IgG antibody. A VDAC3 antibody was used for IB. (E) Comparison of expression levels of FBXW7 in Jurkat, CCRF-CEM, and Reh cells by IB. The expression of β-actin was examined as a control. (F) Histogram of the relative expression levels of FBXW7 under different treatments. Results are presented as means ± SD of three independent experiments. * p < 0.05, ** p < 0.01.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Autophagy Regulates VDAC3 Ubiquitination by FBXW7 to Promote Erastin-Induced Ferroptosis in Acute Lymphoblastic Leukemia

doi: 10.3389/fcell.2021.740884

Figure Lengend Snippet: FBXW7 participated in the ubiquitination and degradation of VDAC3. (A,B) Top 10 E3 ligases of VDAC3. (C) Whole cell lysates were prepared and subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. A FBXW7 antibody was used for IB. (D) Whole cell lysates were prepared and subjected to immunoprecipitation with either the FBXW7 antibody or normal IgG antibody. A VDAC3 antibody was used for IB. (E) Comparison of expression levels of FBXW7 in Jurkat, CCRF-CEM, and Reh cells by IB. The expression of β-actin was examined as a control. (F) Histogram of the relative expression levels of FBXW7 under different treatments. Results are presented as means ± SD of three independent experiments. * p < 0.05, ** p < 0.01.

Article Snippet: The FBXW7 overexpression lentiviral vector (pRRLSIN-cPPT-SFFV-MCS-3FLAG-E2A-EGFP-SV40-puromycin) and FBXW7 RNAi lentiviral vectors (pRRLSIN-cPPT-U6-shRNA-SFFV-EGFP-SV40-puromycin) were purchased from GeneChem (Shanghai, China).

Techniques: Immunoprecipitation, Expressing

FBXW7 affected the ubiquitination level of VDAC3 and the sensitivity of ALL cells to erastin. (A) Confirmation of the transfection efficiency of VDAC3 and FBXW7 proteins in 293T cells with FBXW7 overexpression/RNAi lentiviral vector by IB. The expression of β-actin was examined as a control. (B) Confirmation of transfection efficiency of VDAC3 and FBXW7 mRNA expression in 293T cells with FBXW7 overexpression/RNAi lentiviral vector by qRT-PCR. (C) Histogram of the FBXW7 protein expression with FBXW7 overexpression/RNAi lentiviral vector transfection. (D) Histogram of the relative expression levels of VDAC3 following FBXW7 overexpression or knockdown. (E) Cells with FBXW7 silencing were treated with 10 μmol/l MG132 for 6 h and whole cell lysates were subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. (F) Cells overexpressing FBXW7 were treated with 10 μmol/l MG132 for 6 h and whole cell lysates were subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. A ubiquitin antibody was used for IB. (G) Confirmation of the transfection efficiency of FBXW7 in ALL cells with FBXW7 overexpression/RNAi lentiviral vector by IB. Left, expression levels of FBXW7; right, histogram of results. (H) Confirmation of the transfection efficiency of VDAC3 and FBXW7 in Reh cells with the FBXW7 overexpression lentiviral vector by IB. The expression of β-actin was examined as a control. (H) Trypan blue staining detection of ALL cell death following different treatments. Left, percentages of trypan blue-positive cells; right, histogram of results. p * <0.05, ** p < 0.01, *** p < 0.001, ns. indicates no statistical significance.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Autophagy Regulates VDAC3 Ubiquitination by FBXW7 to Promote Erastin-Induced Ferroptosis in Acute Lymphoblastic Leukemia

doi: 10.3389/fcell.2021.740884

Figure Lengend Snippet: FBXW7 affected the ubiquitination level of VDAC3 and the sensitivity of ALL cells to erastin. (A) Confirmation of the transfection efficiency of VDAC3 and FBXW7 proteins in 293T cells with FBXW7 overexpression/RNAi lentiviral vector by IB. The expression of β-actin was examined as a control. (B) Confirmation of transfection efficiency of VDAC3 and FBXW7 mRNA expression in 293T cells with FBXW7 overexpression/RNAi lentiviral vector by qRT-PCR. (C) Histogram of the FBXW7 protein expression with FBXW7 overexpression/RNAi lentiviral vector transfection. (D) Histogram of the relative expression levels of VDAC3 following FBXW7 overexpression or knockdown. (E) Cells with FBXW7 silencing were treated with 10 μmol/l MG132 for 6 h and whole cell lysates were subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. (F) Cells overexpressing FBXW7 were treated with 10 μmol/l MG132 for 6 h and whole cell lysates were subjected to immunoprecipitation with either VDAC3 antibody or normal IgG antibody. A ubiquitin antibody was used for IB. (G) Confirmation of the transfection efficiency of FBXW7 in ALL cells with FBXW7 overexpression/RNAi lentiviral vector by IB. Left, expression levels of FBXW7; right, histogram of results. (H) Confirmation of the transfection efficiency of VDAC3 and FBXW7 in Reh cells with the FBXW7 overexpression lentiviral vector by IB. The expression of β-actin was examined as a control. (H) Trypan blue staining detection of ALL cell death following different treatments. Left, percentages of trypan blue-positive cells; right, histogram of results. p * <0.05, ** p < 0.01, *** p < 0.001, ns. indicates no statistical significance.

Article Snippet: The FBXW7 overexpression lentiviral vector (pRRLSIN-cPPT-SFFV-MCS-3FLAG-E2A-EGFP-SV40-puromycin) and FBXW7 RNAi lentiviral vectors (pRRLSIN-cPPT-U6-shRNA-SFFV-EGFP-SV40-puromycin) were purchased from GeneChem (Shanghai, China).

Techniques: Transfection, Over Expression, Plasmid Preparation, Expressing, Quantitative RT-PCR, Immunoprecipitation, Staining